Exact(1)
Using images of free and total PSA taken with known concentration values (see, e.g., Figure 5 ), we can then fit standard curves to determine the concentration of PSA for sets of RDT images captured under similar illumination conditions.
Similar(59)
The FL-MoS2 concentration in the upper suspension was determined via the absorption coefficient at 670 nm according to the Beer-Lambert law (A/l = ε C) with a typically known concentration value (C', g L−1).
Since the SEP of both concentrations that are included in calibration and those that are just used for prediction are under 20% of the known concentration value, we show that our calibration set is robust enough for this concentration range.
The standard curve was created using the ABI PRISM 7900 Sequence Detection System software (2.1.1v, Applied Biosystems, Foster City, CA, USA), by plotting the Ct values against each known concentration of the EBV standard.
A standard curve was obtained by plotting the Ct values against each standard of known concentration parasite DNA.
From the measured known concentration of 5.36 × 10 beads mL−1, the threshold value was determined to be 0.023 (Fig. 2a).
The values of Pearson' r between the log2 transformed known concentration and detected read-counts are > = 0.98 (Fig. 1a), indicating high-quality sequencing.
To back-calculate standard concentrations, mean absorbance values of the standards are plotted against the log of the known concentration for each standard.
FRAP values were obtained by comparing the absorbance change at 593 nm in test reaction mixtures with those containing ferrous ions in known concentration.
Accuracy can be measured by analyzing samples with known concentrations and comparing the measured values with the actual spiked values.
In this paper, the correspondence among species in the experiments was used as restriction together with a correlation constraint, in which the concentrations of the analytes in calibration samples at each ALS iteration are forced to be correlated to previously known reference concentration values of the analyte in these samples.
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