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Shotgun proteolysis therefore provides a means to identify domains and other autonomously folding units on a genome-wide scale, without any prior knowledge of sequence or structure.
The accumulation of knowledge of sequence variants over 20 years caused 'consensus decay' of the sites.
They should inform experimental work that is reliant on knowledge of sequence such as the design of primers for PCR reactions and probes for Southern analysis.
Families were obtained by sequence comparison of the catalytic domains, aided by knowledge of sequence similarity and domain structure outside of the catalytic domains, known biological functions, and gross similarity in biological functions of kinases across organisms [7], [28].
Good knowledge of sequence variation is needed for genetics approaches and breeding purposes.
Based on physicochemical properties, PseAAC based features take into account the knowledge of sequence order, achieving a passable prediction performance.
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Another strategy is to sub-divide the probe sets into smaller groups of probes that that interrogate individual exons using prior knowledge of sequences present in transcript databases [26].
These observations, together with the lack of knowledge of sequence-specific methyl-lysine recognition by the L3MBTL1 or Scm MBT-repeat domains prompted us to characterize the MBT-repeat domain of dSfmbt at the structural and functional level.
(c) Scriptlet components constitute knowledge of sequences of activities within particular scenes (Schank, 1999, p. 125).
The main limitation of the array-based approach is that it requires advance knowledge of sequences in target species.
Moreover, the use of universal primers requires no prior knowledge of sequences to design species-specific primers.
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