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In contrast to wild type tissues, neither stretch nor application of L-NAME affecthe the amplitude or frequency of contraction in eNOS knockout tissue strips (Fig. 5B).
(Top and bottom left) wild-type and (bottom right) Gravin knockout tissue.
In Western blots, all three antibodies bound similar proteins in both wild type and P2Y6 knockout tissue.
Glutamine, in contrast, stimulated similar Ca2+ responses in L cells from both wild-type and knockout tissue cultures.
The specificity of our antibody for detecting the endogenous ~28kDa SIRT3 protein was confirmed by using SIRT3 knockout tissue lysates (Supplemental Figure 1).
Using Western blot and IHC on murine wild-type and knockout tissue we have demonstrated that this antibody to ARID1A correctly stains murine tissue by immunohistochemistry. Following reviewers comments, the authors have modified the mansucript to reflect their views.
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Data were collected from three independent labeling experiments, performed twice on two pairs of independent wild-type and knockout tissues, and once on a wild-type mouse tissue.
The increase in proliferation was not accompanied by the loss of differentiated β-cell phenotype since knockout tissues expressed levels of the maturation markers Glut2, Mafa, Mafa and Nkx6.1 similar to those of the heterozygote (supplementary material Fig. S12D).
The specificity of the Shh immunostaining was confirmed using Shh knockout tissues (Fig. 3A,B).
Assessing the mitochondrial respiratory capacities of isoform 1b- and 1d-specific knockout tissues, we found that, in both the cases, several muscle types were significantly affected.
We, at this moment, could not exclude the possibility of pro-inflammatory TLR4 signaling due to missorting of TLR4 in Rab11a knockout tissues.
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