Sentence examples for knockout organisms from inspiring English sources

To generate gene knockout organisms, gene editing using transcription activator-like effector nucleases (TALENs) technique has been introduced to various model animals including worm, cricket, zebrafish, and rat (Wood et al., 2011; Huang et al., 2011; Tesson et al., 2011; Sander et al., 2011; Watanabe et al., 2012).

We chose to use a surrogate organism, E. coli, due to its ease of use and manipulation, and the ability to obtain a knockout organism lacking endogenous S15.

Our data indicate that multiple interaction patterns may exist in vivo, may explain why decorin- or biglycan-knockout organisms show milder effects than can be expected, and may lead to the development of better, simpler engineered biomaterials.

This large number of cellular functions and the lethality in knockout model organisms is in accordance with the severe phenotype previously described in Ogden syndrome patients (MIM 300855).

Thus, in accordance with the observed lethality of homozygous knockout in model organisms a complete loss-of-function of NAA10 seems to be lethal in hemizygous males.

For example, there are numerous single knockouts in model organisms that appear completely normal but a phenotype is observed in double knockouts [ 5].

NITC's reach might extend much further than this, given that gene knockouts in diverse organisms have been shown to produce less-severe defects than corresponding gene knockdowns1.

Knockouts of other organisms have traditionally been made by first using in vitro genetic engineering to modify genes contained on plasmids or bacterial artificial chromosomes (BACs) and later moving these modified constructs to the organism of interest by cell culture techniques.

Despite the different genetic approaches applied, constitutive whole organism knockout [32] and inducible bowel-specific knockout [19, 25]demonstrate all these studies MVID-like phenotypes in mice.

The effect of a gene knockout on an organism's phenotype is frequently used in assessing GSM quality [ 10, 38].

The rational, in silico prediction of gene-knockouts to turn organisms into efficient cell factories is an essential and computationally challenging task in metabolic engineering.