Indole glucosinolate levels were not affected in the myb28-1 myb29-1 double knockout mutant in comparison to the WT or homozygous single knockout lines (Table S14, S15, S16 and S17).
This analysis demonstrated that there was no detectable telomere shortening in TANK1−/− knockout mice in comparison to littermate controls (TANK1+/+) (Fig. 4).
Since already the accumulation of soluble forms of Aβ (monomeric and oligomeric) without plaque or fibril formation is assumed to induce neuronal pathology and consequently learning and memory deficits [12], [19], [20], we tested learning and memory performance in NEP knockout mice in comparison to wild-type animals.
In BAT, β3AR mRNAs were increased in VGF knockout mice in comparison to wild type mice.
A further in vitro approach in studying the P-gp activity is the use of P-gp knockout mice in comparison to wild-type mice.
Behavioral changes in the mdr 1a/1b knockout mice in comparison to the wild-type mice allow for the investigation of P-gp-dependent variations in the expression degrees of the CNS which leads to functional dispositions.
We noted that the width of C3 knockout cortices was reduced in comparison to control (76.4±1.4%, versus 100±3.5%, n=8, Student's t-test, P=0.0004).
However, milk-content in the stomach of knockout mice was less in comparison to control littermates.
The phenotype became apparent in the first weeks of life, several mice died during the weaning period leading to a significantly reduced number of Dgcr8 knockout animals after weaning in comparison to the control genotypes (Additional file 1: Figure S1A).
Furthermore, a recent study showed that knock-in of L435F mutated PS1, which is a loss-of-function mutation, on a PS2 knockout background led to reduced LTP in comparison to PS2 knockouts with wild type PS1 [ 215].
