Sentence examples for knockout chromosomes from inspiring English sources

The transformants were selected in media containing CdCl2 (1 µg/ml at the start of selection and 0.05 µg/ml at the end of selection) and increasing concentrations of paromomycin (from 120 µg to 40 mg/ml) until the WT chromosomes were completely replaced by the knockout chromosomes in the somatic nucleus.

A recent paper reports a similar congenic footprint observation with a Rab3a knockout (chromosome 8) (Yang et al. 2006).

This means that a test male (bearing a rescue transgene) that appears homozygous for the triple knockout chromosome might in fact be heterozygous, having received a recombinant gamete from his (balanced) mother.

As an additional test for genetic background, males that were transheterozygous for a deficiency that removes all three genes (Df 2R Exel6065 ) and the triple knockout chromosome were also tested: they produced on average 22 progeny, a slight but statistically insignificant increase.

The loss of the reporter correlated with the reappearance of the three CG9000 genes as determined by PCR (data not shown), presumably due to recombination in the maternal germline between the triple knockout chromosome and the balancer (there is no meiotic recombination in Drosophila males).

Almost as soon as the triple knockout deletion chromosomes were identified, we began to lose them.

We found that the spindle formation is abnormal in knockout oocytes, and the chromosomes did not cluster together properly.

The efficiency and capacity of the recombineering system were first demonstrated by knocking out various genetic loci on the P. putida chromosome with knockout lengths widely spanning 0.6 101.7 kb.

Recombination of the knockout constructs into the chromosome resulted in replacement of the complete capsule locus or only the siaD gene by an erythromycin- or chloramphenicol-resistance cassette, respectively.

At the same time, POLQ seems to have a unique role in DSB repair that complements the recombination machinery regulated by ATM in HRR since POLQ knockout mice display enhanced chromosome instability in ATM-deficient cells [ 31].

Consistently, the double mutant of rna14-11 and bub1 knockout exhibits high degree of chromosome mis-segregation.