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S.W.P. and H.W.P. provided knockout cell lines.
The MCF10A Arpin knockout cell line, clone #12, was generated with CRISPR/Cas9 system.
In EphB4 +/- knockout cell lines there was a lack of EphB4 inhibition and AKT and ERK showed increased activity.
The activation of EGFR and ERK in PTPRS knockout cell lines and in response to EGF stimulation.
Unlike previous studies, the Vero knockout cell lines failed to achieve desired target yield increases.
a, Numbers of differentially expressed genes in the different knockout cell line models; P ≤ 0.05; these genes are distributed throughout the genome (data not shown).
To generate knockout cell lines, U2OS cells were transfected with PX459 vector containing either PARP2-sgRNA for the PARP2 knockout or APLF-sgRNA for APLF knockout.
The PTPRS knockout cell line in each pair shows the presence of more protein for each product tested than its parental matching cell line.
Subsequently, small-scale models of current industry manufacturing systems were developed and adopted to assess the increases in polio- and rotavirus output by multiple stable knockout cell lines.
Mutants of tRNAAla having diverse sequence combinations in the acceptor-stem region and a broad range of functional activity to support knockout cell growth were isolated.
SSTR levels were measured in multiple, individually cloned FANCC (n = 4) or FANCG (n = 3) knockout cell lines and plotted alongside values for clonally derived wild-type cell lines (n = 5).
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