Serial ultrathin sections from each of the species were cut with a diamond knife, mounted on Cu grids, stained in uranyl acetate and lead citrate, and then observed under a Philips CM 10 (FEI, Eindhoven, The Netherlands) transmission electron microscope (TEM) operating at 80 kV.
Embedded samples were first trimmed with a razor blade and then 2 µm thick sections were cut using a glass knife mounted on an ultrotome.
Thin (∼90 nm thick) sections were cut with a diamond knife, mounted on copper grids, double stained with uranyl acetate and lead citrate [51].
Semi-thin sections (around 2 µm) were cut with a glass knife, mounted on microscope slides, stained with 0.1% boracic toluidine blue and used to study the histology and for localization of appropriate sites for ultrastructural analysis.
Ultrathin (50 70 nm) conventional sections were cut using a diamond knife mounted to a Reichart Ultracut S ultramicrotome.
Semi-thin sections were prepared using a glass knife, mounted on Superfrost Plus glass slides (Thermo Shandon), stained with haematoxylin and eosin and examined as above.
Ultrathin sections (70 nm) were cut using a diamond knife mounted to a Reichert Ultracut S ultramicrotome (Leica, UK) and picked up onto coated EM grids.
Ultrathin tissue sections for TEM were cut at approximately 75 nm with a diamond knife, mounted on copper grids, and stained with lead citrate and uranyl acetate.
Ultrathin sections of 70 nm were cut with a Diatome diamond knife, mounted on pioloform/carbon films on copper slot grids and counterstained with 4% uranyl acetate and Reynolds' lead citrate.
Ultrathin (∼90 nm thick) sections were cut with a diamond knife, mounted on copper mesh grids, double stained with uranyl acetate and lead citrate, and viewed with a Hitachi 7100 transmission electron microscope operating at 100 kV.
Ultrathin sections were cut with a diamond knife mounted in a Reichart ultramicrotome, contrasted with uranyl acetate and lead citrate, and examined in a Jeol 1200 EX transmission electron microscope operated at 80kVV (Brunk et al, 1995).
