The camera should hit the US around the 2nd quarter of 2008 and will likely ship as a kit with one basic lens and other accessories for about $1,300-$1,300-$1,400
TA Cloning Kit with One Shot TOP chemically competent E. coli (Invitrogen, USA) was used for cloning of PCR products.
Short-insert paired-end sequencing libraries were prepared using Illumina (San Diego, California, USA) TruSeq library preparation kit with one modification.
Sequencing was performed by using the dRhodamine Terminator Cycle Sequencing Ready Reaction kit with one of six different primers and AmpliTaq DNA Perkin-Elmer Biosystemss, Warrington, England) with a 3100 ABI Prism automated sequencer (Applied Biosystems, Courtaboeuf, France).
Sequencing was performed on an Illumina GA-II using the Chrysalis 36 cycles v 3.0 sequencing kit, with one lane of 2 × 101 bp reads from both ends of the fragments ("paired ends") with an insert length of > 180 bp.
If multiple PCR products were too similar in size to cleanly separate by cutting, PCR products were cloned using either a TOPO TA cloning kit with one-shot chemically competent cells (Invitrogen) or a pGEM-T easy cloning kit with JM-109 chemically competent cells (Promega).
Total reaction times for the ten real-time assays ranged from 2h42 to 3h08 for two-step kits and 2h18 to 2h57 for one-step kits, with the one-step SSIII Platinium kit requiring the shortest time, and two-step Maxima kit the longest, regardless the machine used.
The $9.99 Strap kit comes with eight straps and the $9.99 Wrap kit comes with one wrap.
PCR amplifications were multiplexed using QIAgen Multiplex PCR kits with between one and five loci run in a single PCR reaction, following the manufacturer's protocol, with the exception that PCR reactions were run in a total volume of 10 μL rather than 50 μL.
The kit comes with one night vision-equipped color camera and an handheld receiver.
