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Testing kits were selected based on an evaluation of available enzyme-linked immunosorbent assay (ELISA) kits in China and comparison with Abbott enzyme immunoassay reagents (Illinois, USA) using a panel of 200 reference serum specimens.
Based on the results displayed in Additional file 1, the combinations of DNA extraction by the ENA kit and WGA by the REPLI-g and GPlex4 kits were selected for further evaluation.
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In practice, test kits are selected and ordered by government officials with no or low experience in HIV diagnosis and selection is often only based on the lower price and not also on tests efficacy.
Cells transfected with WT KIT were selected with 200 ng/mL rmSCF (R&D Systems, Minneapolis, MN, USA).
Among rotavirus antigen positive specimens, specimens that exhibited color intensity at least equal to the positive control provided with the Rotaclone kit were selected for further strain characterization.
The combination of the ENA DNA-extraction with either the REPLI-g or the Gplex4 WGA kit were selected to see if the procedures were robust enough for GWA studies employing numerous samples.
The Johns Hopkins team's product, the Antenatal Screening Kit, was selected from among video entries by university students on three continents who entered a global competition sponsored by ABC News and the Duke Global Health Institute.
This kit was selected, because of its availability and its use in various studies [ 17, 26, 27].
Following pilot purification studies, a monocyte enrichment kit was selected, routinely resulting in >95% purity of CD11b+/F4/8 0+double-positive cells.
Thus, the modified protocol of the large volume kit was selected for further evaluation for SARS-CoV RNA extraction from serum.
Once a kit is selected and internally validated, presumably through a combination of effective marketing and internal validation of performance of a sample kit, the diagnostic laboratory embarks on an adventure that is fraught with even more potential problems.
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