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To examine the differences introduced by different labeling kits, we compared 3 one-cycle labeling kits commonly used with the Affymetrix GeneChip platform.
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In this paper, using HMC-1 (V560G c-Kit) cells we compared NGF and SCF signaling in the same cell system.
We compared three kits specifically developed for the extraction of cfDNA from body fluids (CNA, NS and FA kits) with the DBM kit (which has been used widely for this application [ 18]) using plasma pool A (i).
We compared the kits using the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), Youden index (YI), and negative and positive bacteriology rates.
In the second step, 8E5 cells were used as standards and we compared the kit DNA control value obtained from the LightCycler® quantification with the theoretical value.
In one assay series we compared a commercial ELISA kit for p53 autoantibodies with our in-house ELISA.
The RnD System's IFN-γ ELISA was used as a comparative test against which the different luminex kits where compared.
Regularly, STR results obtained with different PCR amplification kits are compared, for instance with old cases, when revisiting cold cases or when addressing cross-border crimes.
Sensitivity of commercially available CEDIA kits were compared, with respect to the sample and reagent volumes, using three different signal generation processes.
At a recent South Australian rave, results reported to users from on-site pill-testing, using pill-testing kits, were compared with GCMS analysis of a scraping from the same pill.
The plasma progesterone levels indicated by the three EIA kits were compared to one another and to a radioimmunoassay, and assessed for any relationship to the actual time of parturition.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com