On Tuesday, Governor Quinn signed the nation's first state law mandating that all rape kits be tested, as long as "sufficient staffing and resources" are available.
Automated high-throughput extraction comparing several bead-based commercial extraction kits were tested and compared with tissue print and manual extraction to obtain nucleic acids from healthy and pathogen-infected citrus trees from greenhouse in planta collections and field.
The viability of the kits were tested monthly throughout the study period using positive test controls obtained from the manufacturer.
In our experience, no major differences were observed when different silica column- or magnetic beads- based extraction techniques, or different commercialized PCR or RT-PCR kits, were tested (data not shown).
Six commercially available bisulfite conversion kits were tested and the size distributions of converted DNAs were assessed.
An optimized cetyl trimethyl ammonium bromide (CTAB) method (TCM-CTAB) and three commonly-used extraction kits were tested for extraction of DNA from LDW samples.
The sensitivity and specificity of these kits were tested and compared with known standards by using ELISA reagents provided by the Centers for Disease Control and Prevention (Atlanta, GA, USA).
The kits were tested to evaluate the performance of the probes, also in DNA from normal tissue and in DNA from FFPE-derived tissues.
The following commercially available RNA purification kits were tested in this study: mirVana miRNA Isolation Kit (Ambion, Austin, TX), miRCURY Isolation Kit – Biofluids (Exiqon, Inc ., RNeasy Mini Kit (QIAGEN Inc., Valencia, CA), miRNeasy Mini Kit (QIAGEN Inc ., and Direct-zol RNA MiniPrep Kit Zymo Research Corp.., Irvine, CA).
Samples that were reactive with either one or both of these screening ELISA kits were tested again using a third ELISA kit (Monolisa HBsAg ULTRA Assay; Bio-Rad, France) as a surrogate measure for confirmation of HBV infection.
