The Human Biotrak MMP-13 ELIZA kit (GE Healthcare) was used because these kits were previously shown to be suitable for assaying rabbit MMPs [ 28, 29].
VEGF-A and bFGF concentrations in cell supernatant were determined using commercial ELISA kits for humans (Quantikine, R&D Systems, MN, USA) according to the manufacturer's instructions since these kits were previously shown to have cross-reactivity with canine growth factors [ 26, 27].
This Human Cytokine 25-Plex antibody bead kit was previously used to cynomolgus macaque sera and the cross-reactivity was confirmed [ 30].
Genomic DNA samples were purified from cultured fibroblasts and iPSC lines using PureLink Genomic DNA Mini kit (Thermo Fisher Scientific), which was previously used to quantitate mitochondrial DNA copy numbers.57 The quantity and quality of DNA samples were verified by 1.0% agarose gel electrophoresis.
Among other changes we found somatic mutations in ATM, EGFR, EPHA3, EPHB2, and KIT, none of which was previously described in cancers.
However, we were unable to observe any significant expression of c-Kit among the luminal cells isolated from C57BL6/J mice, even when using the same c-kit antibody clone (2B8) that was previously used [ 15].
The diagnostic performance of these kits has been previously evaluated in a Swiss population of RA patients and controls [ 12].
Commercial ELISA kits have been previously evaluated using a selected case series of sera and CSF. 1, 14, 15 However, there is only one published investigation of the accuracy of commercial ELISAs in a prospective study with the clinical description of patients.
The generation of Ba/F3 cells expressing wild-type or mutant (e.g. D816V and V559D) murine and human KIT has been previously described [19], [32].
The antibodies used in the kit have been previously validated to cross-react with bovine IL-8 [ 58].
