For determination of the relative molecular weight of the herein described bacterial PO from the strain A. chroococcum SBUG 1484, native PAGE using a non-denatured protein molecular weight marker kit was conducted.
The sensitivity of kits was 2 pg/mL, and inter- and intra-assay assessments of reliability of the kit were conducted.
In order to minimize the risk of DNA contamination in RNA samples applied for cDNA synthesis and RT-PCR, an additional purification step using the RNeasy Mini Kit (QIAGEN) was conducted.
Extraction by the Qiagen kit method was conducted according to the manufacturer's protocol.
In this context, a multi\e dose-escalating phase I trial to determine the phase II recommended dose of this combination, to determine the impact of oral MC on the IM pharmacokinetic (PK) and to seek some signs of activity in patients with IM and sunitinib-refractory GIST, with chordoma, life-threatening aggressive fibromatosis or melanoma harbouring KIT mutation was conducted.
In order to compare our method to commercially-available RNA amplification kits, microarray analysis was conducted using identical pooled cell line and glioblastoma RNA that was amplified with two commercial products (RiboAmp kit, Arcturus; and BDSmart mRNA Amplification kit, BD Biosciences).
cDNA library construction using the MINT-Universal kit (Evrogen, Moscow, Russia) was conducted per the manufacturer's recommended protocol.
cDNA libraries were prepared for each pooled sample using the Illumina TruSeq RNA Sample Preparation kit and sequencing was conducted using an Illumina GAIIx Genome Analyzer, generating 100 bp paired end reads for the embryonic library and 76 bp paired end reads for 12× multiplexed adult libraries.
Apoptotic lung cells were labeled using the TUNEL staining kit from Chemicon/Millipore (ApopTag® Red In Situ Apoptosis Detection Kit) and PAS staining was conducted using the Periodic Acid-Schiff Kit according to the manufacturer's protocol (Sigma-Aldrich).
Serum total Hp was determined using a commercially available bovine Hp ELISA kit.e The analysis was conducted according to the manufacturer's instructions.
