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The drugs industry was strictly controlled at the time, but diagnostics kits using the new technologies of gene sequencing were not yet regulated.
ELISA tests were achieved using Concateno Cozart Microplate kits using the recommendations of the manufacturer.
The cytokine secretion in cell culture supernatants was measured by ELISA kits using the manufacturer's protocol (BioSource International, Camarillo, CA).
These lipid levels were assessed with commercial kits using the VITROS-800 machine.
We compared the kits using the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), Youden index (YI), and negative and positive bacteriology rates.
Larval ticks were finely minced with scissors prior to extraction with DNeasy blood and tissue kits using the Qiagen supplementary protocol for the purification of DNA from ticks to detect B. burgdorferi sensu lato (43).
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All kits used the OC125 antibody.
Both kits use the same phage Φ29 DNA polymerase to amplify the DNA templates, although the buffer components are proprietary.
The reagents were supplied in the form of chromogen, enzyme-buffer and standard kit using the method of Koracevic et al. (2001) for total antioxidant capacity.
Bob Somers has put together a PC app that lets you play an electronic drum kit using the Wiimote (though, he kind of sucks at it).
ATPase assay used the ADP-Glo Kinase Assay kit using the manufacturer's guidelines (Promega Inc).
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CEO of Professional Science Editing for Scientists @ prosciediting.com