Morning, fasting cortisol levels were measured in participant's plasma samples using a Liaison Cortisol kit (Reference 313261, DiaSorin, Italy).
Purified DNA samples were amplified using the DiversiLab Escherichia DNA fingerprinting kit (reference number: 410 980, bioMérieux) on a T-personal thermal cycler (Biometra, Göttingen, Germany).
β-hemolytic, catalase-negative, Gram-positive cocci were classified to Lancefield group (A, B, C, F, and G) using the PathoDX Strep Grouping Kit (reference 62076; Remel).
Total RNA was extracted from the PBMCs lysates (1×10 or 3×10 cells) using the miRNeasy mini kit (reference 217004, Qiagen, CA), the Total RNA Purification Kit (product 17200, Norgen Biotek Corporation, Canada) and the NucleoSpin miRNAs kit (reference 740971, Macherey-Nagel, Düren, Germany) following the manufacturers' protocols.
Total RNAs were extracted from thawed serum samples using the miRNeasy mini kit (Qiagen, CA), the Plasma/Serum Circulating RNA purification Kit (product 30000, Norgen Biotek Corporation, Canada) and the NucleoSpin miRNAs Plasma kit (reference 740981, Macherey-Nagel, Düren, Germany).
Up to two mucosal respiratory specimens (throat and/or nasal swabs) were collected for testing using Dacron swabs (Remel, Inc., MicroTest Viral Transport Media [VTM] Female Kit, Reference #12550) immediately placed into the VTM.
The kit references arguably Sir Patrick's most famous acting role of Jean-Luc Picard, Captain of the USS Enterprise in Star Trek: The Next Generation, adopting his uniform's maroon colour and gold studs signifying his Starfleet rank.
Which is why Kaltura has launched software development kits and reference applications that will make getting in front of users easier, regardless of whatever device they're trying to watch a video on.
EarlyTox Cardiotoxicity Kits, including reference standards isoproterenol, propranolol, and sotalol, and CatchPoint cAMP GPCR assay kits were purchased from Molecular Devices, LLC (Sunnyvale, CA).
We assessed the quality of the sequences obtained by 454 GS-FLX Titanium sequencing, using the control DNA fragment Type I sequences (provided with 454 sequencing kits) as reference templates (see Materials and Methods for details).
