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The measurement was performed by using ARA-L kits obtained from ABCD GmbH and a chemiluminescence measuring device HP-CLA (Tohoku, Japan).
The concentration of alanine aminotransferase (ALT) and aspartate transaminase (AST) in serum were assayed with the commercial kits obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu, China).
Blood samples were tested in 3 different ELISA kits obtained from Cayman Chemical Ann Arborr, MI, USA), GenWay Biotech (San Diego, CA, USA) and Calbiotech (Spring Valley, CA, USA), respectively.
Insulin and leptin was assayed by RIA using kits obtained from Linco Research (St. Charles, MO).
A total of 115 MAM treated embryos were obtained from 16 pregnant ferrets and 27 normal ferret embryos or kits obtained from 13 pregnant ferrets.
Plasma levels of Pf4, Igf1, Igfbp5, and Lcn2 were measured using commercially available mouse DuoSet kits obtained from R&D Systems (Minneapolis, MN).
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We used end-repair kit obtained from Epicenter [37] in order to add the complementary bases on the 3′ ends one blunts the 5′- ends.
ChIP analysis was performed using the ChIP Assay Kit obtained from Upstate (Lake Placid, NY).
Urine albumin levels were measured using a kit obtained from Nanjing Jiancheng Bioengineering Institute, China.
U-c-AMP was measured using a kit obtained from R & D Systems, Minneapolis, MN, USA.
D-Dimer measurements were performed using a kit obtained from Kamiya Biomedical Company (Seattle, WA, USA).
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