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For a wide range of algal samples, obtaining a higher yield of purified genomic DNA proved difficult using classical methods such as CTAB, the Dellaporta protocol, or commercially available DNA purification kits (not shown).

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All the other tests of the API 20E kit not shown in the table resulted negative for all the strains.

Almost all of the germ cells in these clusters were immunopositive for OCT4 (Fig.  2B), AP2γ and C-KIT (not shown), indicating that they were undifferentiated gonocytes.

Immunostaining of serial testis sections with AMH (Fig.  2A), OCT4 (Fig.  2B), AP2γ and C-KIT (not shown) confirmed the presence of unusual clusters of germ cells in the two aforementioned MBP-exposed animals.

Staining pattern and positive-to-negative signal ratio [i.e., staining index (SI)] of the 259D/C7 (PE-CF594) clone were most optimal with the BD TF kit (not shown) and were comparable to the staining pattern of the 206D clone using this kit, indicating that both antibodies could be used in our Treg panel (Supplementary figure 1d f).

However, when using the thermocycler Rotor Gene MQDx, the real-time one-step SYBR Green assays with universal pan Lyssavirus revealed a high presence of Primer-Dimers for three out of five one-step kits tested especially when RNA concentration was low (below 100 copies/ µL) compared to two-step kits (data not shown).

The basolateral concentration of these cytokines was below the detection threshold of ELISA kits (data not shown).

Either preserving procedures, chemical addition and other phases of immunoglobulins purification did not affect the avidity and specificity of antibody binding; indeed, immunoglobulins from HIV-positive specimens conserved their anti-HIV reactivity when assayed in commercial ELISA kits (data not shown).

We never achieved transfection efficiencies above 10%% using these kits (data not shown).

Plasma IL-2, IL-4, and IL-13 were not detectable by the ELISA kits, thus not shown.

No SSA and SSB antibodies were detected in serum from any strain at any age by using these kits (data not shown).

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