"It had the same font and was called Naked Eye Kit instead of Naked," Ms. Zomnir recalled of their version.
To eliminate any remaining DNA, the mixture was treated by 1 U of DNase I (Ambion, USA) for 15 min at 37°C and purified by use of RNeasy MinElute Cleanup Kit (QIAGEN, Germany) according to manufacturer's recommendations, except for using RPE solution (included in the Cleanup Kit) instead of 80% ethanol in the second washing step and performing the elution step twice, each with 15 µl of water.
The 8 kb size selected library fragment was purified using a Qiagen Gel Purification Kit instead of the Elutrap (Whatman, Piscataway, NJ).
Tissue sections containing cervical cancer were used as positive controls for p16INK4a, while negative controls consisted of incubated doublet slides in the negative control reagent contained in the kit, instead of primary antibodies.
Recent advances allow us to assess the functional complement activity (FCA) of the three pathways independently, and in parallel, by using the novel ELISA (Wielisa®-kit) instead of a hemolytic assay.
For example, midway through NHANES III (1988 1994), the NCEH began to use the Bio-Rad Quantaphase II assay kit, instead of the Quantaphase I, to measure serum and RBC folate (20).
At the screening visit, a cytological smear was taken with an Ayre's spatula, and cervical cells were collected with the sampler brush included in the HC2 test kit (instead of a regular cytobrush).
You may also want a custom kit instead of a factory vehicle's kit.
You may be able to paint over your kit's pieces or use a kit instead of paint.
But because of the dusty interior and the swarm of cables and electrical boxes, I kept thinking of the 918 Spyder prototype as a kit car instead of the state-of-the-art machine that will cost as much as four Bentley Continental GTs with enough left over to buy a Panamera S. Undoubtedly, this perception will recede as the 918 takes on its final polished form.
