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The cytokine levels were determined by enzyme-linked immunosorbent assay (ELISA) using commercial kits for human IL-1β, IL-6, interleukin-10 (IL-10), TNF-α, and interferon-gamma (IFN-γ), according to manufacturer's instructions (eBIOSCIENCE, San Diego, USA).
Immunoassay kits for human parathyroid hormone (hPTH) quantification from three different vendors were assessed in a side-by-side comparison for their suitability for the PK analysis of recombinant humanPTH (rhPTH) in EDTA plasma.
ELISA kits for human IL-12p70 and human IFN-γ were obtained from eBiosciences.
The enzyme immunoassay kits for human TNF-á detection and for human IL-6 detection were from R&D (Lille, France).
ELISA kits for human TNF-α and human and mouse IL-6 (DuoSet ELISA development systems) were obtained from R&D Systems.
Cytokine levels in the supernatants were determined by using ELISA kits for human IL-1b, IL-6, IL-12, IL-17, IFN-γ (R&D systems), and IL-23 ELISA (eBioscience) according to the manufacturer's instructions.
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VEGF-A and bFGF concentrations in cell supernatant were determined using commercial ELISA kits for humans (Quantikine, R&D Systems, MN, USA) according to the manufacturer's instructions since these kits were previously shown to have cross-reactivity with canine growth factors [ 26, 27].
First aid kits for humans and horses.
Sequenom launched the first commercial SNP typing kit for human identification, named the iPLEX® Sample ID Plus Panel.
The CGRP content of the samples was determined using a commercial sandwich enzyme immune assay kit for human CGRP, which is crossreactive to mouse CGRP (SPIbio, France).
An ELISA kit for human IL-6 was from R & D Systems (Minneapolis, MN).
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