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For children 5 years of age or older, parents and/or older children were also asked to take two additional DBS samples at home 4 weeks apart, dry them overnight at room temperature and mail them to our laboratory in prepaid mailer kits for analysis.
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The specificity and method accuracy in comparison with commercial PSA ELISA kit for analysis of human serum specimens were relatively satisfactory.
The practical aspects and efficiency of the CE-IVD-marked Pyrosequencing kit for analysis of KRAS mutations have until now not been evaluated in a clinical setting.
Faced with the need to analyse a large number of plasma samples obtained from subjects undergoing acute tryptophan depletion and loading tests, we successfully applied the above kit for analysis of the relevant plasma AA using GC-FID.
We used SYBR Green PCR Kits (Applied Biosystems) for analysis.
ELISA kits for quantitative analysis of mouse TNF-α, IL-6, adiponectin, leptin and FAS, rabbit anti-IR-α, goat anti-rabbit IgG (γ-chain specific) and goat anti-mouse IgG (γ-chain specific) were from Wuhan Boster Biotech Co., Ltd (Wuhan, China).
Calpro AS supplied the CalproLab ELISA kits for the analysis of calprotectin levels.
JUJ has fulfilled speaking engagements and received payments for this from the Brahms company, that makes kits for procalcitonin analysis.
Acetylcholine chloride, cholesterol, cholic acid, phenylephrine, potassium chloride and tyloxapol (triton WR-1339), were obtained from the Sigma Chemical Company, St . Louis MO, U.S.A. Randox diagnostic kits for serum analysis obtained from Randox Laboratories Ltd., Co. Antrim, UK.
RNA was extracted using the RNeasy mini kit (Qiagen) for analysis by qPCR of MT2A and MT3 gene expression levels.
The secondary PCR product was purified and inserted into pMD19-T vector (TaKaRa Cloning kit) for sequence analysis.
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