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All extraction reagents were included with all kits, except for ethanol added for wash steps.
All hormones were assayed in duplicate using specific commercial RIA kits, except for catecholamines (high-performance liquid chromatography).
The y-intercept varied between 36.80 and 40.74 for all tested kits except for the qScript kit (Intercept of 40.74).
We were informed by the manufacturer that there was no difference between the BCII RUO and BCII IVD kits, except for the label.
The 4 isolates did not ferment most of the carbohydrates in the 2 API kits, except for ribose, maltose, glucose, and sucrose.
A threshold setting of 300 was used as the reference for the Mx3005P thermocycler for all tested kits except for one two-step kit (Express) for which a threshold setting of 0.05 was used.
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The concentrations for 148 metabolites (all analytes were determined with the metabolomics kit except for the amino acids, which were determined by a different method) obtained by internal calibration were exported for comprehensive statistical analysis.
All had received but not completed a bowel cancer screening kit except for one focus group participant who had recently entered the eligible age range and had not yet received a test kit.
All operations were conducted according to the instructions of each kit, except for the following: (1) If there is a bead-beat step, the Fast bead beater (FastPrep@-24, MP Biomedicals, USA) was adopted for all kits with the setting at 6.0 m s−1 and 5 × 1 min duration; (2) the volume of the elution buffer was 100 μl in the final elution.
The remaining eleven cases displayed a band similar to the positive control on electrophoresis, and analysis confirmed the sequence was identical to the wild-type exon 11 of c-KIT except for a single nucleotide polymorphism (SNP) located at base pair 50110905 C > T [GenBank: NC_006595.2] detected in four cases.
The qPCR cycling protocol was the same for both kits, except that the initial denaturation step was 95°C for 5 min for the QVK and 95°C for 10 min for the TUMM.
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CEO of Professional Science Editing for Scientists @ prosciediting.com