Recently, we decided to move toward a more sensitive method, the Mutaquant kit (Ipsogen), characterized by a detection limit close to 0.1%.
C3a and C5a ELISAs are "ready-to-use" kits and are characterized by BD Bioscience.
We decided to use the Gaudí kit to characterize RSCs functionally in a mature retina.
These tests were used in parallel with more traditional specialized culture medium and a commercial serotyping kit to characterize Cryptococcus isolates from AIDS patients in southern California.
Specificity and sensitivity of the anti-KIT antibody and efficacy of the staining protocol were confirmed by the relevant staining patterns observed in the characterized KIT positive and negative control tissues.
Mutations in coding or regulation of KIT have been characterized in additional species as causative of defects in pigmentation and hearing (Haase et al. 2007; Ruan et al. 2005; Spritz and Beighton 1998).
To ensure specificity of the antibody, appropriate controls, such as uterine tissue (negative control), GIST (characterized KIT positive case) from the clinical collaborators and commercially purchased normal meningeal tissue sections (BioChain, Hayward, CA, USA) were included for processing in each batch.
The internal tandem duplication mutations are the most frequent and best characterized c-KIT mutations in canine MCTs [ 35, 38].
Recently, in our own work we have also isolated and characterized c-kit positive CSCs from adult hearts [ 12, 13].
In our study, in descriptive manner, we characterized c-Kit+ cells as cells displaying mesenchymal stem cells features with mostly endothelial specification.
We have characterized c-kit positive cardiac stem cells after long-term cultures and established a 3D-nanoculture system using collagen scaffolds.
