Sentence examples for kits binding from inspiring English sources

In AITDs patients, thyroid autoantibodies (anti-thyroglobulin and anti-thyroid peroxydase) were measured by ELISA and indirect immunofluorescence using commercially available kits (BINDAZYME™ Human EIA kits, Binding site Ltd ,Birmingham, UK) with the respective normal ranges of 0 to 100 and 0 to 70 IU/mL.

While most residues in the direct SCF/c-Kit binding interface can be identified from the existing crystal structure of the complex, other residues that affect binding through protein unfolding, intermolecular interactions, allosteric or long-distance electrostatic effects cannot be directly inferred.

When docked into the KIT binding site, the aminothiazole of masitinib participates in a hydrogen bond with the side-chain of the gatekeeper residue Thr670.

A molecular docking simulation showed that hesperidin, a major component of KMP6, controls the SCF and c-kit binding by interaction with the active site of the c-kit.

Our results show for the first time that KMP6 potently regulates SCF-induced migration, p38 MAPK activation and inflammatory cytokines production through hindrance of SCF and c-kit binding in RPMCs.

It is thought that the D816V mutation interferes with the interaction of imatinib and its KIT binding site.

Briefly, 10 μl of 30-35 mg/ml protein solution was adsorbed to 10 μl of beads after addition of 20 μl kit binding buffer.

Nilotinib treatment, using low concentration (i.e., 20 nM) specific to BCR-ABL inhibition, was associated with a loss of c-KIT constitutive activation, restored in a large part after c-KIT binding by SCF.

Library construction and subsequent sequencing were performed using the SMRTbell Template Preparation Reagent Kit 1.0, DNA/Polymerase binding kit P4-C2, MagBead Kit and DNA Sequencing Kit 2.0 (all components supplied by Pacific Biosciences, Menlo Park CA, USA).

According to the protocols of the kit, the binding reaction was carried out in a total volume of 20 mL containing ultrapure water, 10× binding buffer, 1 μg/μL Poly (dldc), 50% glycerol, 1% NP-40, 100 mM MgCl2, unlabeled p-CREB probes/unlabeled mutational p-CREB probes, protein extract (15 μg/lane), and biotin-labeled p-CREB probes.

Several potentially useful tyrosine kinase inhibitors with distinct inhibitory profiles against both KIT ATP-binding domain and activation loop mutations have not yet been fully evaluated.