We obtained similar results using our homemade kit (QuatroG) or the commercial kit at different times after treatment (6 h or 12 h) and concentrations of cisplatin (20, 40, 80 and 160 μg/mL), indicating that purified rhANXA5 is functionally active.
The linear relationship between [Ca2+] and (F − Fmin) / (Fmax − F) has been previously confirmed by calibrating the indicator as expressed in neurons, exposing them to ionomycin in the presence of sequentially different solutions of precise [Ca2+], obtained by mixing K2EGTA and CaEGTA solutions (Calcium Calibration Buffer Kit, Invitrogen) at different ratios [82].
The expression of IgM, C3, TGF-β1, IL-1β, transferrin, lysozyme and their concentrations were detected respectively with quantitative real-time PCR and Elisa kits in offsprings at different stages from both naive and vaccinated brood stocks.
Fasting glucose assessments were obtained with a One Touch Ultra Smart kit at two different time points: 90 and 172 days of age.
To our knowledge, there are only two studies assessing the introduction of an all-inclusive central line kit at two different intensive care units [ 10, 18].
They have intrinsic proliferative capacities and express Kit and EpoR at different levels.
Cell numbers were detected with the CCK-8 kit (Dojindo, Japan) at different times after incubation.
The secretory Gaussia princeps luciferase (Gluc) activity was measured using BioLux® Gaussia Luciferase Flex Assay Kit (New England Biolabs) at different time point during the experiment.
The finding of KIT mutations in only 2 of 56 SNMMs suggests that KIT mutations differ between mucosal melanomas at different sites, and that they are very rare in this subtype of mucosal melanomas.
Mixtures of the animal species the kit is designed to detect were prepared at different concentrations and tested on raw/pasteurized, and heated meat and milk matrices.
