Two APS kits were processed per patient.
Positive and negative controls from the workflow control kit were processed with each batch of samples.
The nitrocellulose membranes provided with the kit were processed and developed as per the manufacturer's instructions.
"The 14 days that it was supposed to take for my kit to be processed turned into 868 days," Marlowe told the San Francisco Police Commission during a meeting in 2013.
For the CNA kit, samples were processed according to the manufacturer's protocol for 1-mL input volume with an elution volume of 50 μL.
As a modification to the protocol of the DNeasy Blood and Tissue kit, samples were processed in a spin/rotation instrument for cell lysis (FastPrep-120), with a speed and time setting of 6 and 45 seconds, respectively.
Next, crude membranes that were extracted using commercial kits (KIT 1 and 2) were processed by single-FASP.
WOMAC surveys were completed at baseline and at follow-up visits throughout the study, and two APS Kits (nSTRIDE Autologous Protein Solution (APS) Kit, Biomet, USA) were processed per patient.
Immediately after dehydration and staining (HistoGene, LCM Frozen Section Staining Kit, Arcturus), slides were processed in an Autopix Laser Capture Microdissection instrument (Arcturus) and cancer cells captured on CapSure MacroCaps.
Immediately following dehydration and staining (HistoGene, LCM Frozen Section Staining Kit, Arcturus), slides were processed in an Autopix (Arcturus) instrument for laser capture microdissection (LCM) of cancer epithelial cells (CEPI), cancer stroma (CS), and normal stroma (NS) using CapSure Macro-LCM Caps (Arcturus).
