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Regularly, STR results obtained with different PCR amplification kits are compared, for instance with old cases, when revisiting cold cases or when addressing cross-border crimes.
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Sensitivity of commercially available CEDIA kits were compared, with respect to the sample and reagent volumes, using three different signal generation processes.
At a recent South Australian rave, results reported to users from on-site pill-testing, using pill-testing kits, were compared with GCMS analysis of a scraping from the same pill.
The plasma progesterone levels indicated by the three EIA kits were compared to one another and to a radioimmunoassay, and assessed for any relationship to the actual time of parturition.
Several parameters of these one-cycle labeling kits were compared to determine factors that could affect gene expression results.
The proportion of bases in Known Gene regions captured by different versions of CCDS-based capture kits was compared.
Five different systems for the generation of cDNA, five two-step PCR kits, and five one-step qRT-PCR kits were compared.
The quantity and quality of RNA extracted from each of these kits was compared in an effort to determine which kits were most appropriate for low- and high-throughput RNA extractions of small tissues.
The following RT kits were compared: Iscript (IScript cDNA synthesis kit, Bio-Rad, France), QuantiTect (Quantitect Reverse transcription kit, Qiagen, France), Maxima (Maxima H Minus First Strand cDNA synthesis kit, Fermentas, France), SS Vilo (Superscript Vilo Master Mix, Invitrogen, France), and qScript (qScript cDNA Synthesis kit, Quanta, France).
The value of Spearman's ρ calculated for each sub-populations of positive or negative results, showed that there was an association between ranks when the two blocking kits were compared as could have been expected given they were based on the same blocking ELISA design.
Here it is reported from a comparative study where HER2 gene status obtained by HER2 CISH pharmDx™ Kit was compared to HER2 gene status obtained by the FDA approved HER2 FISH pharmDx™ Kit and the PathVysion HER-2 DNA probe Kit.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com