Sentence examples for kits additional from inspiring English sources
Exome libraries were prepared using Agilent SureSelect, Illumina TruSeq and Nextera exome capture kits (Additional file 14) following manufacturers' specifications.
Detailed instructions for blood and saliva sample collection were mailed together with the sample collection kits (Additional file 1).
For DNA isolation we employed commercial DNA tissue kits (Additional file 4: Table S2) following the manufacturer instructions.
Additionally, we describe the effect of varying conditions (pH, temperature and protein concentration) when labelling a His-tagged protein with the IsoLink kit (Additional file 1: Figure S1).
After four weeks of culture, differentiation of wt and P2rx7−/− BMMCs was comparable according to expression of FcεRI and Kit (Additional file 2B).
The IC50s were 73.5 μM for Abl (Additional file 1a) and greater than 100 μM for c-Kit (Additional file 1b) and concentrations significantly above the maximal plasma concentrations of GW2580 achieved in mice receiving 80 mg/kg GW2580.
All specific miRNA forward primers were designed based on the mature miRNA sequence, while the reverse primers were the adaptor sequence provided in One Step Prime-Script miRNA cDNA Synthesis Kit (Additional file10).
For miR-221, our results validated 4 out of 10 of the miR-221 targets predicted by the prediction software [ 10– 12], and an additional 4 out of 9 of the targets previously validated by others (WEE1, ETS2, FMR1 and KIT) (Additional file 2: Table S2) [ 42– 42].
Primers for site-directed mutagenesis were designed using the PrimerX program (http://www.bioinformatics.org/primerx/) under the following conditions: melting temperature >78 °C and primer termination with guanine (G) or cytidine (C) for QuickChange site-directed mutagenesis kit (Additional file 1: Table S3).
Treated or untreated cells were analysed for apoptosis using the M30 CytoDEATH™assay kit (Additional file 1(Table S1) for source), based on binding of an antibody to a Caspase-cleaved epitope of cytokeratin 18-cytoskelatal protein in apoptotic cells and not in viable or necrotic cells (Peviva AB, Bromma, Sweden).
To further assess accuracy of the API 20 STREP kit, additional catalase-negative gram-positive coccoid or coccobacillary isolates during 2005 2006 with vancomycin MIC ≥ 256 μg/ml were also evaluated by the same phenotypic and genotypic assays (isolates 31 38 in Table 1).
