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All the cases were extracted using a Qiagen kit, whereas the controls were extracted using a salting-out procedure.
The results were consistent between both reference assays and suggested that the yield of cfDNA per millilitre of plasma was between 2.2-fold and 2.5-fold higher with the CNA kit than with the DBM kit, whereas the NS extracts contained less than half the cfDNA yield of the DBM extracts.
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However, a closer examination showed that 100% of the embryo samples extracted via the Plus Universal kit, whereas only 25% of the samples isolated by the Plus Mini kit contained, contained >1 μg of total RNA.
The single Border Collie sample was extracted without the NaCl step via the beta kit instructions, whereas the ten Bearded Collie samples were all extracted using the NaCl step from the updated protocol.
Caspase 12 activity was determined using a Caspase-12 Fluorometric Assay Kit (BioVision), whereas the activities of caspase 3/7, 8 and 9 were determined using luminescence Caspase-Glo Assays (Promega, Madison, WI, USA) according to the manufacturer's instruction.
The adaptor ligation was performed using reagents from the same kit, whereas barcoded adaptors were retrieved from the Ion Xpress™ Barcode Adapters (Life Technologies Inc., Waltham, MA, USA).
Also, all investigated flocks were bulk milk antibody negative in the CHEKIT Q fever ELISA kit, whereas one herd, located in the county of Västerbotten, showed a reaction in the ELISACox kit.
All 30 HCV-positive reference serum samples were detected as positive with the HRP-LD5 assay and with the Abbott 3.0 kit, whereas one was not positive, according to the two domestic kit assays.
Of the 3121 members eligible for randomization, 1559 were assigned to the 1-FIT group (receiving one FIT kit), whereas 1562 were assigned to the 2-FIT group (receiving two FIT kits).
Participants showed most interest in the medical and health section of the Youth KIT whereas other popular sections were those about personal and school information.
Libraries 1 and 2 were constructed using the ClonTech SMART cDNA kit, whereas libraries 3, 4 and 5 used a modified version of the kit: the CDS III/3' primer we used was (5'-TAGAGGCCGAGGCGGCCGACATGTTTTGTTTTTTTTTCTTTTTTTTTTVN) to avoid long homopolymer repeats unsuitable for 454 sequencing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com