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In the late 1990s, the kit was estimated to be used in only 1% of cases [ 16].
Hence the number of women who were offered EHC, who should have been given a screening kit, was estimated from routine audit data collected by the PCTs for the EHC programme.
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The total cost of the RDT intervention over this period (not including the test kits) was estimated to be $16,946 in 2008 USD or $1,883 USD per implementing facility.
Serum NGAL (Rat NGAL ELISA Kit, Bioporto Diagnostics, Gentofte, Denmark), creatinine, and BUN (standard kits) were estimated to evaluate renal function.
For trials hoping to increase the return of postal questionnaires with chlamydia test kits, the number of kits returned was estimated to increase by 33 per 1000 sent when GBP20 was offered as an incentive, rather than GBP10 (95% CI 11 to 54).
Template DNA was extracted from skeletal muscle using the QIAmp Tissue Kit (Qiagen); concentration was estimated by comparison to standards using the intensity of ethidium bromide fluorescence.
Kit diagnostic sensitivity was estimated using sera collected from 609 infected reference ewes after six months of milk production (Table 2).
The half-life of the KIT protein in each group was estimated after plotting KIT protein expression relative to the untreated control.
Protein concentration for each sample was estimated with BCA kit (Perbio, Brebières, France).
T-Tf (ELISA) was estimated with Human Transferrin ELISA kit (Alpha diagnostic international Inc., Cat # 1210) following the manufacturer's instructions.
Finally, the protein content was estimated using BCATM Protein Assay kit (Pierce) and the endotoxin level in the protein was measured using QCL-1000 Endpoint chromogenic LAL assay kit (Lonza).
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