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The presence of KIT was determined using Western blot.
The precision of the therascreen IDH1/2 kit was determined using a protocol based on the CLSI EP05-A2 [ 25].
The sensitivity of each kit was determined as a minimum rate of CFU providing the positive result in the PCR assay.
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The analytical specificity and sensitivity, intra- and inter-assay repeatability and diagnostic characteristics of the kit were determined and compared with virus isolation, which is the gold standard.
Plasma concentrations of glucose (Sopachem, Ochten, the Netherlands), triglycerides (TG), cholesterol (Instruchemie, Delfzijl, the Netherlands), glycerol (Sigma-Aldrich, Houten, the Netherlands) and free fatty acids (Wako Chemicals, Neuss, Germany; HR(2) Kit) were determined following the manufacturers' instructions.
Finally, the activity of bound enzyme in all kits was determined with tetramethylbenzidine/hydrogen peroxide substrate.
The concentration of RNA isolated with RNeasy kits was determined by measuring the absorbance at 260 nm in a UV-spectrophotometer.
Dr. Hamedanizadeh explains that distribution of the kits is determined by the size of the affected population and the number of Rural Health Units in the area.
KIT activation was determined in total protein extracts prepared from 17 frozen GIST samples by two different methods.
Presence of c-KIT mRNA was determined using reverse transcription polymerase reaction (RT-PCR).
The RNA cargo of the exosomes was analyzed with a Bioanalyzer and absence or presence of the c-KIT mRNA was determined by RT-PCR.
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