Sentence examples for kit volume from inspiring English sources

Four different recombinant His-tagged proteins (recombinant complement receptor 2 (rCR2) and three single chain antibodies, α-CD33 scFv, α-VCAM-1 scFv and α-PSMA scFv), were used to study the effect of kit volume, ionic strength, pH and temperature on radiolabelling of four proteins.

In method 3, we further reduced the kit volume by subdividing the kit into 260-μL freeze-dried aliquots, each able to reduce up to 1 GBq [99mTcO4]- in 100 μL resulting in a radioactivity concentration of approximately 8 GBq/mL after neutralisation.

DNA was extracted from 300 µL whole blood using MagNA Pure LC DNA Isolation Kit – Large Volume on the MagNA Pure LC (Roche), eluted and diluted to 1 mL, of which 5 µL was applied in each assay.

20 µL of each neutralized Catch-2 eluate was transferred to a new 96-well plate and 5 µL of 10x Agilent Block (Oligo aCGH/ChIP-on-chip Hybridization Kit, Large Volume, Agilent Technologies 5188 5380), containing a 10x spike of hybridization controls (10 Cy3 SOMAmers) was added to each well.

Viral RNA was extracted from 0.5 mL of culture supernatant with the same kit (elution volume 50 μL).

After determining its protein concentration using Bio-Rad DC protein assay kit, equal volume of 2× sample buffer [0.1 M Tris (pH 6.8), 2% SDS, 0.2% β-mercaptoethanol, 10% (v/v) glycerol, and 0.0016% (w/v) bromophenol blue] was combined with the cytoplasmic extract.

DNA was extracted on the MagNA Pure instrument using the MagNA Pure LC DNA isolation kit-Large Volume, in accordance with the manufacturer's instructions.

These data suggest that the modified protocol of the large volume kit has comparable performance with the total nucleic acid kit for the extraction of samples containing high SARS-CoV concentration, but enabled more sensitive detection when samples containing low levels of SARS-CoV were extracted.

We showed that the use of the large volume kit resulted in better analytical sensitivity when compared with the total nucleic acid kit as evident by the higher rates of positive detection among samples containing low concentrations of SARS-CoV.

Furthermore, when the sample contained 10 copies/mL SARS-CoV, the large volume kit yielded positive detection in four replicates, while viral RNA extractions from the total nucleic acid kit was only positive in one replicate.

Extracted nucleic acids were then purified using the EZ1 Virus Mini Kit v2.0 (elution volume: 90 µl) onto the EZ1 Biorobot (both from Qiagen).