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After kit installation, a "kit check" sequence is initiated to assure that the kit has been properly installed.
Libraries were quantified with KAPA qPCR library quantification kit, and sequenced on an MiSeq or HiSeq Sequencers (Illumina) using 150 bp or 125 bp paired-end sequencing protocols.
Enzymatic primer digestion before sequencing makes better utilization of sequencing throughput.
Clone libraries of the PCR-amplified rDNA with these primers were constructed using a pCR2.1 TA cloning kit and sequenced.
Amplified PCR products were purified using GenElute™ PCR Clean-up kit and sequenced at Eurrofins Genomics India Pvt Ltd., Bangalore.
We followed the manufacturer's protocol of SMART-Seq v4 Ultra Low Input RNA kit for sequencing (Clontech #634889).
Targeted sequence enrichment was performed using the Agilent SureSelect Target Enrichment Kit and sequenced on the Illumina HiSeq2000 using paired-end reads.
PCR products were inserted into the TA sequencing kit for sequencing (Invitrogen).
mRNA libraries were prepared according to the TruSeq RNA Illumina kit protocol and sequenced using a HighSeq 2500 sequencing system (Illumina).
resulting PCR product was cloned into pCRII-TOPO vector (Invitrogen) using TOPO TAcloning kit (Invitrogen) and sequenced.
Ultra Low RNA Kit for Illumina Sequencing, Protocol PT5163 1, version PR0X3693 (Clontech Laboratories, 2011).
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