Sentence examples for kit step from inspiring English sources

The samples were extracted following the exact instructions in the protocol supplied with the kit (step 9 to 17).

Digested samples were eluted into a clean collection tube via three steps of centrifugation at 14,000 × g for 15 min as follows: Step 1: 80 μL 50 mM ammonium bicarbonate solution; Step 2: 50 μL 0.5 M sodium chloride solution (provided with the FASP kit); Step 3: 170 μL H2O with 0.1%% formic acid.

"We've been a different football club, on and off the pitch, since Kit stepped in as our caretaker manager.

For a one-off fee of €45 (£35), they can provide a digital start-up kit with step-by-step advice on everything from finding local repair experts, choosing a suitable locations, collecting the right tools, generating publicity and funding your project.

This introductory kit provides step-by-step instructions along with a few of the materials one needs to repair battered, torn and smudged books at home.

9. We introduced you to the Kano Computing learn-to-code kit, a step-by-step hand-holding layer atop the Raspberry Pi single-board microcomputer to make hacking around with code and learning about computational thinking child's play.

This model-based kitting practice reduces − even eliminates − the need for a manual kit preparation step and promises additional supply chain benefits.

The relative mRNA levels of SOD, CAT, GSHPx, MT, HSP70, CYPA, P53, GST, and TF were determined using a real-time quantitative PCR (qPCR) kit (One Step SYBR® PrimeScript™ RT-PCR Kit, PR066A, Takara, Dalian, China).

Biolegend kit: One Step Staining Human Treg Flow Kit (FOXP3 Alexa Fluor 488/CD25 PerCP4 PerCP) was used.

Finally, each sample was further purified with the Qiagen QIAquick PCR purification Kit (see step 2) according to the manufacturer's protocol.

Total RNA was extracted by using QIAamp viral RNA mini-kit (QIAGEN, Crawley, UK), converted to cDNA, and amplified by using a single-step kit (Superscript III one-step RT-PCR with Platinum Taq polymerase system; Invitrogen, Paisley, UK) in conjunction with oligonucleotides arena1+ and LCMV322– (10 ) or 1010C and either OW1696R or NW1696R (8 ).