Sentence examples for kit obtained from from inspiring English sources

We used end-repair kit obtained from Epicenter [37] in order to add the complementary bases on the 3′ ends one blunts the 5′- ends.

The cells were then lysed with lysis buffer and the relative Luciferase activity was measured using the Luciferase assay reagent kit obtained from Promega (Madison, WI).

The sections were washed in PBS to remove excess antibody and developed using the M.O.M. and peroxidase DAB kit, obtained from Vector Laboratories (Burlingame, CA).

MMP-2 levels were determined using an ELISA kit obtained from R&D Systems (Minneapolis, MN, USA) according to the instructions of the manufacturer.

cGMP content was assayed as described in the procedure of an ELISA kit obtained from Cayman Chemical & Co. cGMP level was normalized to tissue protein content in mg/ml.

ChIP analysis was performed using the ChIP Assay Kit obtained from Upstate (Lake Placid, NY).

Next, avidin-biotin assays were done using the Vectastain Elite kit obtained from Vector Laboratories.

Cellular ATP concentrations were measured with ATP Bioluminescent Somatic Cell Assay Kit obtained from Sigma (Sigma, St . Louis MO, USA).

The ability of Ctx, PD173074, and Ctx+PD173074 to induce apoptosis was assessed using the Cell Death Detection ELISAPLUS Kit obtained from Roche Diagnostics (Barcelona, Spain).

Total RNA was extracted and first-strand complementary DNA was synthesised using the RNeasy Micro Kit obtained from Qiagen (Valencia, CA, USA) from each EC type.

Activity of hepatic PTP was determined using PTP ELISA assay kit obtained from Sigma, USA, according to a method based on the in vitro colorimetric determination of protein tyrosine phosphatase (PTP) activity [ 26].