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Four micrograms of the mRNA were used in the 5′-RACE-Ready cDNA and 3′-RACE-Ready cDNA synthesis using Clontech's SMART RACE cDNA Amplification Kit (Mountain View, CA).
Biovision's Triglyceride Quantification Kit (Mountain View, CA) was used to assay for triglyceride content.
The nuclear and cytoplasmic proteins were prepared using the Biovision Nuclear/Cytosol Fractionation Kit (Mountain View, CA) as per manufacturer's instructions and used in further analyses [43].
The transcriptome was constructed from a 5th instar larva using the Clontech SMART™ PCR cDNA Synthesis Kit (Mountain View, CA, USA), following the 'first-strand cDNA synthesis' and 'cDNA Amplification by LD PCR' instructions.
Total RNA was prepared from the BT474 cell line and Stratagene Universal Human Reference RNA (StratRef) (Stratagene, La Jolla, CA) using the Arcturus PicoPure RNA isolation kit (Mountain View, CA) as per manufacturer's instructions.
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All reactions were performed with kits according to the manufacturer's instructions (Smart RACE cDNA Amplification Kit, Clontech, Mountain View, CA, USA).
Apoptosis was measured using the Annexin V-FITC apoptosis detection kit (Abcam, Mountain View, CA, USA) according to the manufacturer's instructions.
Cytochrome c release was determined using the cytochrome-c-releasing apoptosis assay kit (BioVision, Mountain View, CA, USA) according to the manufacturer's protocol.
The amplified 2.83-kbp fragment of CE0634-CE0635 was fused to BglI-digested pCH using the InFusion Cloning Kit (Clontech Laboratories Inc ,Mountain View, CA, USA).
We used a commercial G-6-P assay kit (Biovision Research Products, Mountain View, CA).
The cDNA library was constructed using the Creator™ SMART™ kit (Clontech Laboratories Inc., Mountain View, CA, USA).
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