Sentence examples for kit method from inspiring English sources
The traditional screening kit method and a multifactorial approach were compared against each other with regard to their ability to find new crystallization conditions that are compatible to the purification process of a recombinant aprotinin variant.
In DNeasy blood and tissue kit method, de-proteinization was achieved by proteinase K. Proteinase K is active in the presence of SDS and also at elevated temperature (56 65 °C).
A non-His-tagged protein (C2Ac [12]) was allowed to compete for radiolabel with a His-tagged protein (α-CD33 scFv) by incubating an equimolar mixture of both proteins (55 μM:55 μM) in a one-pot radiolabelling reaction using the IsoLink kit method 2 described above.
cDNA synthesis was performed by the Titan One Tube RT-PCR Kit method (Roche Applied Science).
Samples underwent a freeze/thaw cycle 4X between liquid nitrogen and a 37°C waterbath followed RNA isolation using the Qiagen RNeasy Lipid Tissue kit method with the addition of the RNase free DNase steps (Qiagen).
DNA extracted from blood samples by Qiagen kit method.
The results indicated that the developed assay and commercially available kit methods were highly correlated.
The method used was significantly associated with the occurrence of perforation (p value = 0.014) where BAOSFE was associated with the largest number of perforations (58.4%, n = 7) compared to 8.3%and8.3%3% for the balloon and CAS kit methods, respectively.
Following electroblotting to 0.2 μ nitrocellulose for 60 volt hours, the membrane was blocked with subsequent immunoblotting using OxyBlot Kit methods and reagents.
Sera from all patients were then screened for the presence of HIV antibodies: using the WHO recommended strategy for HIV antibody testing using two kit methods.
To evaluate whether sequence divergence could affect exome capture, especially in a mixed genetic background, we performed exome sequencing on a F1 hybrid mouse derived from crossing C57BL/6 J and SPRET/EiJ mice using an Agilent SureSelect XT Mouse All Exon Kit (Methods).
