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Bacterial DNA was extracted with ATP™ Genomic DNA Mini Kit (ATP Biotech Inc , USA according to the kit manual.
Masson trichrome staining was performed following the manual of Trichrome Staining Kit manual.
The treatments described below were applied on cells grown in BMGH medium (pH 4.5; Invitrogen EasySelect™ Pichia Expression Kit manual) supplemented with 1/50 (v/v) of a solution containing 250 mM MnSO4 and 50 mM MgSO4.
The collected spores were grinded in liquid nitrogen, and the mRNA of T. virens ZY-01 was extracted with RNAprep Pure Plant Kit (Tiangen Biotech, Beijing) according to the kit manual.
The PCR analysis was carried out using the ABI PRISM7500 Fast Real-Time PCR System as described in the SYBRPremix Ex Taq™ (perfect real time) (TAKARA, Japan) kit manual.
Post-transcription purification steps mirrored those given by the MEGAscript RNAi kit manual.
Total RNA isolation then proceeded as directed by the RNAqueous® Kit manual.
Duplex-specific nuclease (DSN) treatment was performed as described in the Evrogen kit manual.
Per sample, 500 ng total RNA was amplified according to the Agilent QuickAmp kit manual (Agilent technologies).
Chromatin immunoprecipitation (ChIP) assays were performed according to a modified protocol from the acetyl-histone H3 immunoprecipitation kit manual (Upstate).
All cellular samples were immediately subjected to the lysis buffer and further processed with RNA extraction according to the RNeasy Mini Kit manual (Qiagen, UK).
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