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The worms were homogenized, and the supernatant was collected and measured using an ATP determination Kit (Life technology) and the Glucose Oxidase Assay Kit (Life technology).
Plasmid DNA was extracted from bacterial culture using Quick Plasmid Miniprep Kit (Life technologies).
The libraries were quantified using Qubit dsDNA BR assay kit (Life technologies, USA).
mRNA was isolated using the FastTrack MAG mRNA isolation kit (Life technologies, Carlsbad, CA, USA).
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To generate iPSCs, fibroblast cells were transfected with a Sendai virus reprogramming kit (Life Technologies, A16517).
gRNA-1 transcribed using the MEGAshortscript T7 kit (Life Technologies) following the manufacturer's instruction.
Total RNA was extracted from cells with PureLink RNA kit (Life Technologies Corporation).
mRNAs and gRNAs were subsequently purified using the MEGAclear kit (Life Technologies) and resuspended in RNase-free water.
Visualization was achieved either using Coomassie Brilliant Blue staining or Pierce® Silver Stain Kit (Life Technologies, Darmstadt, Germany).
HF2-BE2 mRNA was transcribed using the mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies) following the manufacturer's instruction.
mRNAs and gRNAs were subsequently purifed using the MEGAclear kit (Life Technologies) and resuspended in RNase-free water.
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