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The homogenates were centrifuged at 15000 g for 15 min at 4°C, and the supernatants stored at −20° C. Cytokine content was evaluated using the BD mouse inflammation cytometric bead array (CBA) kit (BD, Basel, Switzerland) or using a DuoSet ELISA kit for determination of IL-1β (R&D systems).
Kit for determination of creatinine and UN were purchased from Span Diagnostic Ltd.
The supernatants were then subjected to ELISA using the CST PathScan Sandwich ELISA kit for determination of phosphorylated and total VEGFR2 according to manufacturer's instructions.
Following termination of experiment, cell culture supernatants were harvested and subjected to Sandwich ELISA using the R&D quantikine ELISA kit for determination of secreted VEGF according to the manufacturer's instructions.
The diagnostic kit for determination of plasma levels of high density lipoprotein cholesterol (HDL-C) was purchased from Bio-Quant Diagnostics (Cat. no. BQ 019CR; San Diego, CA, USA).
This study is the first to demonstrate a clinical usefulness of RASKET KIT, a CE-marked and approved by the Ministry of Health, Labour and Welfare of Japan in vitro diagnostics (IVD) kit for determination of all RAS mutation status in FFPE tissues of CRC patients.
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The new Gyrolab huIgG Kits for determination of human immunoglobin G (IgG) come in two configurations, high titer and low titer, and have been developed to accurately and robustly quantify intact human IgG during cell line development.
Kits for determination of plasma levels of total triglycerides (TG) was purchased from Cayman Chemical Company.
Kits for determination of MR-proADM and MR-proET1 were provided by Brahms Hennigsdorff, Germany).
Secondly, we used human kits for determination of TNFα and for coagulation tests, due to the lack of kits specifically designed for sheep.
Enzyme-linked immunosorbent assay (ELISA) kits for determination of the levels of IL-6, IL-10 and TNF-α were purchased from eBioscience (San Diego, CA, USA).
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