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Leuko, S., Legat, A., Fendrihan, S. & Stan-Lotter, H. Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples.
Fixed and permeabilized cells were blocked with 10% horse serum in PBS and incubated with primary antibodies in blocking solution followed by secondary antibodies together with click-it kit for detection of EdU and DAPI for nuclear staining.
In order to evaluate the FP method for detection of HBV infection, ELISA experiment was carried out using a commercial ELISA kit for detection of IgG of anti-HBV.
The IMAGEN kit for detection of Chlamydia was from DAKO HamburgGermanyany).
ELISA kit for detection of mouse sTnfsf14 was from Cloud Clone Corp. (Houston, TX, USA).
Direct colorimetric immunoassay kit for detection of cAMP was purchased from Merck Biosciences, Nottingham, UK.
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The objective of the study was to compare the performance of 3 different extraction instruments in conjunction with 4 different amplification and detection kits for detection and typing of human papillomavirus (HPV) deoxyribonucleic acid (DNA).
Luminex kits for detection of rat IL-2, IL-4, IFN-γ and IL-10 were from Bio-Rad (Hercules, CA).
The manufacturing facility was designed to produce two types of diagnostic kits: Hepcell kits for detection of hepatitis B virus, and Kemcom kits for detection of HIV in the blood (both based on particle agglutination technology).
Commercial ELISA kits for detection of PCBs and most OCPs are available from Millipore Corp. (Billerica, MA, USA) and Strategic Diagnostics (Newark, DE, USA).
ELISA kits for detection of anti-MCV were generously provided by ORGENTEC Diagnostica GmbH (Mainz, Germany) and were used in accordance with the manufacturer's instructions [ 9] with the recommended cut-off value of 20 U/ml.
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