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Both cell lines were tested for Mycoplasma contamination with a Hoechst staining kit (Flow, Tokyo, Japan).
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(E ) Quantification and statistical analysis of CD71/Ter119 and CD71/c-Kit flow cytometric analysis.
Apoptotic cells were measured using paraformaldehyde fixed cells with an APO-DIRECT kit (Phoenix Flow Systems) with flow cytometry or the Cell Death Detection ELISAPLUS (Roche Applied Science) according to the manufacturer protocol.
All cells were processed using the APO-bromodeoxyuridine staining kit (Phoenix Flow Systems, San Diego, CA, USA), and subjected to a flow cytometry-based, modified TdT-mediated dUTP nick end labelling (TUNEL) assay as described previously (Sueoka et al, 2000).
Cell cycle and apoptosis were examined by an Annexin V-FITC/propidium iodide (PI) kit with flow cytometry (FCM).
HMME-based PDT induced macrophage apoptosis via caspase-9 and caspase-3 activation pathway detected by caspase fluorescent assay kit and flow cytometer.
Caspase 3 activity was determined using CaspGLOW Fluorescein Caspase-3 Staining Kit by flow cytometry.
Murine cell culture supernatants were analyzed by a CBA mouse Th1/Th2/Th17 kit by flow cytometry using a FACSCalibur (Beckton Dickinson) according to manufacturer's instructions.
Apoptotic cell death was measured using a flouresin isothiocynate (FITC -conjugated Annexin V/PI assay kit by FITC -conjugated
Dead cells were identified using LIVE/DEAD Fixable Aqua Dead Cell Stain Kit for flow cytometry (Life Technologies).
Assay performed according to the manufacturer's instructions (Annexin V-FITC Apoptosis Detection Kit, Phoenix Flow Systems, San Diego, CA, USA).
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