Sentence examples for kit eBioscience from inspiring English sources
Tregs were analyzed by using mouse regulatory T cell Staining Kit (eBioscience).
For apoptosis analysis, after surface-marker staining, BM cells were washed and stained with Annexin V/DAPI, using an Annexin V Apoptosis Detection Kit (eBioscience or BD Pharmingen).
Cells were fixed and permeabilized for intracellular staining using a Fixation/Permeabilization kit (eBioscience, San Diego, CA, USA) at +4 °C for 30 min.
After this period, the medium was collected and the concentration of TNF-α and IL-1β was measured by Instant ELISA kit (eBioscience, Vienna, Austria) according to the manufacturer's manual.
Foxp3 staining was performed using a commercially available kit (eBioscience).
For FoxP3-staining, a commercially available kit (eBioscience) was used.
Foxp3 staining was performed using the Mouse Regulatory T-Cell Staining Kit (eBioscience, San Diego, CA).
Intracellular Foxp3 staining was performed utilizing the Fixation and Permeabilization Kit (eBioscience) according to manufacturer's instructions.
Intracellular Foxp3 expression with an Intracellular Foxp3 Detection kit (eBioscience) was done according to the manufacturer's instructions.
Intracellular staining for Ki67 was performed using the FoxP3 staining kit (eBioscience) according to the manufacturer's instructions.
