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The kit components were determined after the PCR amplification conditions were serially optimized.
The EEG kit components are discarded and sent out for refurbishment.
The Invitrogen One-Step SYBR Green RT-PCR (Invitrogen) kit components were used for the PCRs.
A pegboard mounting platform is standardized on Lego spacing, so that Make to Learn Invention Kit components can be combined and intermixed with Lego parts.
As students develop an understanding of basic building blocks, they gain an ability to recombine Invention Kit components in an infinite number of ways as they design and construct their own inventions.
Radiolabeling of 5 μg (5.3 nmol) PSMA-HBED-CC in 3 mL 1.5 M HEPES (Kit components, ABX, Radeberg, Germany) was performed during 10 min at 95 °C in a reaction vessel for single use from the kit.
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Active Ras was detected using a Ras antibody (kit component) or an isoform specific antibody to K-ras or N-ras (both Santa Cruz).
Secondary antibodies were diluted as follows: FITC-conjugated goat anti-mouse IgG (1∶200), Alexa Fluor 633-conjugated goat anti-mouse IgM (1∶300), Alexa Fluor 546-conjugated streptavidin (1∶750) and Alexa Fluor 488 Signal-Amplification Kit component A (1∶80).
Table 1 provides a detailed description of the contents, utilization and evidence surrounding each kit component.
The pellet was gently re-suspended in 55 μl of solution #1 (kit component).
A detailed description of each kit component, its intended utilization and associated evidence is outlined in Table 1.
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