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A RNA integrity number (RIN) was determined for each sample using an Agilent Bioanalyzer 2100 and the RNA 6000 Nano Kit Chip (Agilent Technologies Inc., Santa Clara, CA).
The RNA quality for each sample was determined by RNA integrity number (RIN) using an Agilent Bioanalyzer 2100 and the RNA 6000 Nano Kit Chip (Agilent Technologies Inc., Santa Clara, CA) with 250 ng of total RNA per sample.
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ChIP-qPCR was performed using the ChIP-IT High-Sensitivity Kit and ChIP-IT qPCR Analysis Kit (Active Motif, USA) according to the manufacturer's instructions.
Templated libraries were then sequenced on Ion Torrent Proton™ sequencing system, using Ion PI kit and chip V2.
"You can point your finger at some of the kit manufacturers – chips can be designed to be more resilient, lots of devices can be shielded.
RNA was checked for purity and degradation by capillary electrophoresis using the Bio-Analyzer Experion (Bio-Rad; RNA Standard Sens kit; RNA StdSens chips).
ChIP assay was performed using the ChIP kit (Upstate Biotechnology, Lake Placid, NY) according to the manufacturer's protocol.
ChIP was performed using the LowCell#ChIP kit (Diagenode) with modified wash conditions (see methods in the supplementary material).
ChIP was carried out using EZMagna Protein A ChIP Kit from Millipore according to the manufacturer's instructions.
ChIP assay was performed with the EZ-Magna ChIP kit (Millipore) according to the manufacturer's instructions with minor modification.
ChIP assays were done as previously described [17] using the ChIP kit (Upstate Biotechnology, Millipore, Lake Placid, NY, USA).
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