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In two years, the GP Kit, according to my friend, has become a collector's item.
The cDNA was obtained through reverse transcription kit according to manufacturer's instructions (TOYOBO, Japan).
Protein concentration was determined using the PIERCE BCA protein assay kit according to the manufacturer's protocol.
Total RNA was isolated with the Qiagen Mini Plus Kit according to the manufacturer's protocol.
and total DNA isolated using the same kit according to the manufacturer's instructions.
Protein levels were quantified using a Pierce ™ BCAprotein assay kit according to the manufacturer's recommendations.
Total RNA was extracted using a TRIzol kit according to the manufacturer's instructions (Invitrogen, http://www.invitrogen.com/).
THE DOWNTOWN LA STARTER KIT According to Patton Oswalt.
Luciferase activity was assayed using the Dual Luciferase Assay kit according to the manufacturer's instructions (Promega).
RNA was extracted from biopsies using QIAGEN RNeasy extraction kit according to the manufacturer's instructions.
Silver staining was done using the BioRad Silver Stain kit, according to the manufacturer's recommendations.
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