"You can wear a sensor in your shirt, on your wrist, shoe or raquet; we're even seeing sensors in golf clubs to monitor players' swings using kinetic real-time feedback".
Fig. 6 Graphical representation of the kinetic real-time cell-binding approach.
Data of the observed association phases determined in kinetic real-time cell-binding studies were transformed to start at time point zero.
Kinetic real-time binding studies with [18F]FE@SNAP were conducted on adherent CHO-K1 cells stably expressing the human MCHR1 and MCHR2.
Kinetic real-time cell-binding studies were conducted on CHO-K1-hA3R cells with [125I]-AB-MECA at ambient temperature with LigandTracer® Grey.
Furthermore, we successfully established in kinetic real-time cell-binding studies an experimental design for the effective and reliable quantification of the rate constants k on and k off in order to determine the K d of a radiolabelled ligand.
Kinetic real-time cell-binding experiments were performed to determine the K d of the radiolabelled A3R agonist [125I]-AB-MECA based on the quantification of the rate constants k on and k off.
In addition, a new kinetic real-time cell-binding approach was established to quantify the rate constants k on and k off, as well as the dedicated K d of the A3R agonist [125I]-AB-MECA.
Although previously published work already addressed determination of k on, k off, K d, K i and B Max [19 22], in the present kinetic real-time cell-binding studies, we established an alternative experimental design for the assessment of the rate constants k on and k off.
The K d of [125I]-AB-MECA obtained from the novel kinetic real-time cell-binding studies and from traditional binding assays, as previously reported in the literature (see Table 2 for detailed summary of K d values), was compared using two-tailed, unpaired Student's t test with Welch's correction (d).
Synthesized cDNA was analyzed by quantitative kinetic real-time PCR using the ABI Prism 7700 Sequence Detector system (Applied Biosystems) with SYBR Premix Ex Taq (Takara) [38], [39].
