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To the best of our knowledge, there is no report on the use of induction period effect of TU for its kinetic determination and so this is the first report on the kinetic determination of TU based on induction period on the MCP-bromate-sulfuric acid as a novel reaction system.
The use of the Fenton's reagent (FR) (a mixture of H2O2 and Fe2+) for the kinetic determination of individual chemical species is proposed.
Based on the literature survey only a few numbers of indicator reactions for the kinetic determination of TU by spectrophotometric method have been published (Abbasi et al. 2009; Abbasi et al. 2010).
MCP has been widely used in analytical chemistry in acid base titrations, the kinetic determination of inorganic substance such as bromide (Ensafi et al. 2004a), nitrite and nitrate (Ensafi et al. 2004b) and organic analyts such as piroxicam (Arab Chamjangali et al. 2012) and phenylhydrazine (Arab Chamjangali et al. 2009).
For kinetic determination, the O2 concentrations were varied between 0 and 9 vol.% for reduction, and between 21 and 1.5 vol.% for oxidation reactions; temperature was varied between 1148 and 1273 K for the reduction and between 1123 and 1273 K for the oxidation.
This paper explores two issues in greater depth the optimum selection of heating rates for kinetic determination based on statistical error considerations and the importance of using a diverse set of thermal histories, including but not limited to isothermal and linear heating, to better constrain and validate global models.
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For the kinetic determinations, assay linearity was confirmed under all conditions.
Limitations in analyte solubility precluded concentration-dependent kinetic determinations in rat liver cytosol.
The quantitative assessments presented above were performed as end point assays on fixed cells, yet they are perfectly suitable for repeated measurements and kinetic determinations on living cells.
Although O2 uptake was the first method that we considered, the slow turnover for FIH makes high-precision kinetic determinations by this method challenging.
The final incubations for the kinetic determinations contained 0.10 unit of XO/mL (CalBiochem), varied concentrations of 1, N-ε-Gua, and 0.4% DMSO in 0.8 M potassium phosphate (pH 7.6).
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