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The kinetic chromogenic Limulus Amebocyte lysate test was adapted to measure endotoxin activity.

Currently, the most sensitive test for detecting endotoxins is the kinetic chromogenic Limulus Amebocyte lysate (LAL) test.

Methods: For endotoxin testing the quantitative kinetic chromogenic LAL assay Kinetic-QCL, (Lonza Group Ltd ,Basel, Switzerland) with a 96 well pyrogen-free microplate was used.

Overall, there are only a few references on in vitro endotoxin binding studies which were done using the kinetic chromogenic LAL test, and most of these studies were done with other sorbents than clay minerals.

Endotoxin was measured using the Limulus Amoebocyte Lysate kinetic chromogenic assay.

Endotoxin was analyzed using the kinetic chromogenic Limulus Amebocyte Lysate assay (Lonza, Inc., Walkersville, MD) as previously described [ 6].

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The endotoxins concentration of the purified M-hMPV was determined by a kinetic chromogenic-QCL Bio Wittaker Limulus amoebocyte lysate assay at Laboratoire Marcel Mérieux (Lyon, France) and was negative (<0.25 U endotoxins/mg of M-hMPV protein).

Endotoxin was analyzed using the kinetic quantitative chromogenic Limulus amoebocyte lysate (KQCL) method (Chun et al. 2002).

Free LPS in rumen fluid was measured by a chromogenic kinetic Limulus amebocyte lysate (LAL) assay (50 650U, Kinetic-QCL, Lonza Group Ltd., Basel, Switzerland) in a 96-well microplate using an incubating microplate spectrophotometer (Synergy H4 Hybrid Multi-Mode Microplate Reader, Bio-Tek, Instruments, Inc., Winooski, VT, USA).

Lastly, endotoxin concentration was measured using a chromogenic kinetic limulus amebocyte assay (LAL assay, BioWhitaker, Walkersville MD), which had been validated, previously [ 21].

Thrombin activity was determined by a kinetic assay using the chromogenic substrate Biophen CS-21 66 CS-21 66lu-pyro-Glu-Pro-Arg-pNA∙HCl0 μM) at 415 μM concentration.

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