Josh kindly drove me to the airport in the morning.
In this mouse, the flag- FoxN1 cDNA (kindly provided by Dr. Brissette) driven by a pCAG promoter (kindly provided by Dr. McMahon) was inserted into the Rosa26 locus.
"He seemed very nice," I said to one of his young assistants, who had kindly offered to drive me back to my hotel.
He had a great 1989, playing a Civil War hero in "Glory," the baseball-bat-wielding high-school principal Joe Clark in "Lean on Me" and Jessica Tandy's kindly chauffeur in "Driving Miss Daisy".
I guess I must have been ahead of him on day one – until I stopped off at 3am for a couple of hours' sleep in the motorhome my brother Tim had kindly agreed to drive.
Reporter plasmid 5XGAL4-LUC and internal control pPTRL (Renilla reniformis Luciferase driven by 35S promotor) were kindly provided by Dr. Masaru Ohme-Takagi.
Probasin promoter driven luciferase-reporter plasmid (pGL3E-Probasin) was kindly provided by Prof. Dr. Z. Culig (Innsbruck, Austria).
Plasmid pAR3126 encodes T7 polymerase driven by an SV40 early promoter [71] and was kindly supplied by William Studier (Brookhaven National Laboratory, Upton, New York).
Mouse Fox1 and Fox2 cDNAs were kindly provided by Dr. Kawamoto [39] and cloned into the Gateway Destination vector driven by a CAGGS promoter.
The plasmid reporter used was the luciferase reporter gene driven by the p53-dependent natural promoter Noxa (Noxa-luc; kindly provided by T. Taniguchi, University of Tokyo, Japan).
